Biotechnology techniques Assignment Example | Topics and Well Written Essays - 1500 words

Biotechnology techniques - Assignment Example Is this statement TRUE or FALSE, explain? (5 points) Electroporation is used to introduce DNA in prokaryotic and eukaryotic cells. This is the general method of introducing the foreign DNA into the desired cells. This method uses the electrical pulses to create a pore in the cell membrane and through these pores the foreign DNA molecule is inserted. 3. What are the 3 main sources of Stem Cells? (10 points) The 3 main sources of stem cells included Bone marrow, Bloodstream or peripheral blood and finally umbilical cord blood from the newborns. Bone marrow particularly those in the pelvis’ bones have rice supply. Blood normally has few stem cells, and when hormone like substances called growth factors are given to individuals, stems cells grow faster and move from the marrow to the blood. Blood left in the placenta and the umbilical cord of the newborns can be retrieved, store and utilized for future.   4. What is meant by humanization of mouse antibodies and what is the need for "humanization"?  (10 points) While using therapeutic antibodies or murine antibodies isolated from mice in humans, immunogencity problem arose due to the differing protein sequence. To alleviate this issue, specific regions within a mouse antibody called complementarity determining regions (CDR) are identified and transferred into human variable regions and this process is called humanization. When this is done, it will confer binding and so the resultant humanized antibodies can function without major immunogencity problem.   5. Which technology cannot be used to determine the function of a gene? Explain. (5 points) Stem cell technology cannot be used to study the gene function because, it is a method to produce the entire cell and study the organs and it is not the direct method of analysis of gene function.    6. cDNA microarray technology specifically evaluates the gene expression levels of ONLY microRNA in cells and tissues. Is this statement TRUE or FALSE? Explain? ( 5 points) No cDNA microarray technology is used for the analysis of all the genes and its function. cDNA technology is used to analyse the functions of known and unknown gene.    7. Which of the following gene expression strategies increase the number of different proteins available to the cell without increasing the total number of genes encoded by the genome?(5 points) Translation is used to increase the number of different proteins in the cell without increasing the total number of genes in the genome. By varying the mRNA translation, the protein synthesis is modified.    8. In the sequence given below, how many EcoRI recognition sites are present? Please circle them. (10 points)   Ã‚  Ã‚  Ã‚  Ã‚  5’-ATGGAATTCCCAATTGAATTCCGGCTGAATTCAAA-3’   Ã‚  Ã‚  Ã‚  3’-TACCTTAAGGGTTAACTTAAGGCCGACTTAAGTTT-5’ There are three EcoRI recognition sites in the given sequences. They are   5’-ATGGAATTCCCAATTGAATTCCGGCTGAATTCAAA-3’   Ã‚  Ã‚  Ã‚  3â⠂¬â„¢-TACCTTAAGGGTTAACTTAAGGCCGACTTAAGTTT-5’ 9. What is meant by the statement, "Complementation screening of genomic eukaryotic clones will not work in E.coli because of absence of splicing machinery?" (10 points) E.coli is a prokaryote. The bacterial systems cannot remove the introns from the mRNA sequences. Complementation screening is the technique used to identify the mutations in the gene of interest. In this method, the selection is based on the capacity of the gene to get back the phenotype. The splicing mechanism is not present in the

Increasing use of Medical Marijuana Essay Example | Topics and Well Written Essays - 1250 words

Increasing use of Medical Marijuana - Essay Example So, the medicines developed from cannabis are also known as psychoactive drugs. So, these drugs are offered high attention by the young generations of this era as compared to many other medical drugs (Politics. Co.UK. n. d). Cannabis is recognized as marijuana. Cannabis or marijuana drugs are mainly used as a stimulant to increase the appetite of the body, improve the mood or relaxation of the entire body. So, it is also recognized as recreational drugs and excess intake of these drugs might cause negative impacts over the health of the body such as anxiety, reddening of the eyes, dry mouth, loss of memory and many others. Therefore, due to these sorts of side effects, it is illegal to carry or consuming a small amount of cannabis or medical marijuana in any of its form. But, in-spite of implementation of numerous rights and regulations of the government, the use of cannabis or medical marijuana is increasing at a rapid rate among the individual of all age groups and all income groups (Politics. Co.UK. n. d). Cannabis or medical marijuana is considered as one of the most illicit drug in the entire globe, but legalizing its use for medical purposes created a considerable challenge for all. Due to such legalization, the usage rate of medical marijuana increased mainly among the youth adults of age-groups, 12 to 20 years. However, the rate of consumption of medical marijuana is also extremely high in the nations comprising of numerous rules and laws against its consumption rather than those, without comprising any laws. Therefore, due to increased rate of consumption of medical marijuana, the rate of crimes, alcohol consumption, loss of memory and deaths increased to a significant extent as compared to previous era. According to a research, conducted by Moir & et. al. (2008), it might be clearly depicted that the smoke of cannabis or marijuana comprises of more than 15,00 harmful and toxic chemicals such as carbon monoxide, irritants, hydrogen cyanide,